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To construct expression vectors for the secretion of Exe4, double-stranded DNAs of Exe4 fused with signal peptides (SPs) and furin-cleavable sequences were synthesized (Azenta) and inserted into a CAG promoter-containing pCAG backbone vector using the In-Fusion HD cloning kit (Takara Bio)
Hausner, Helene, Julie Derving Karsbl, Anders G
8 GIPR deficiency in mice leads to impaired glucose tolerance with reduced cell function, 9 while GIP overexpression results in improved glucose tolerance, enhanced cell function, and resistance to highfat dietinduced obesity
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High fat diet feeding induced insulin resistance exacerbates 6-OHDA mediated neurotoxicity and behavioral abnormalities in rats